Introduction to Cryo-electron microscopy, 3 hp, 3 hp

Beskrivning

PhD course in single-particle cryo-EM for imaging and structure determination of macromolecular complexes (proteins and nucleic acids). The main focus of the course will be projects based on real samples where the students will freeze, screen, collect data and process the data. This will be complemented by lectures, seminars and tutorials.

Email course responsible for registration information (late signup/waiting list available).

Inlärningsmål

After completion of this course, students will have gained:

- understanding of the theory behind cryo-EM
- insight into practical applications of this technique, the role it plays in structural biology and science, and how it pertains to their own research
- insight into the basic principles of cryo-EM sample preparation and optimization
- understanding of methods for data collection, image processing, 3D reconstruction and model building
- hands-on experience in preparing samples for cryo-EM analysis
- hands-on experience in operating the cryo-EM
- hands-on experience in using various computational methods to process data and generate atomic models

Innehåll

- Transmission electron microscope optics
- Fourier transforms and image formation in transmission electron microscopy
- Principles of biological sample preparation and optimization for cryo-TEM
- Electron microscopy operations and methods for data collection
- Principle methods of cryo-EM image processing, 3D reconstruction and map validation

Undervisning

- Lectures
- Labs (wet labs and computer labs)
- Student presentations
- Student-led seminars

Examination

Project presentation

Lärare

Daniel Larsson m.fl.

Kontakt

Daniel Larsson, daniel.larsson@icm.uu.se

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