Introduktion till kryo-elektronmikroskopi, 3 hp

Beskrivning

Doktorandkursen i singelpartikel kryo-EM för avbildning och strukturbestämmning av makromolekylära komplex (proteiner och nukleinsyror). Kursen är till största delen projektbaserad där deltagarna arbetar med riktiga prover som de fryser, screenar, samlar data på och analyserar. Projektet kompletteras med föreläsningar, seminarier och övningar.

Kontakta kursansvarig för registreringsinformation, efteranmälan och väntelista.

Inlärningsmål

After completion of this course, students will have gained:

- understanding of the theory behind cryo-EM
- insight into practical applications of this technique, the role it plays in structural biology and science, and how it pertains to their own research
- insight into the basic principles of cryo-EM sample preparation and optimization
- understanding of methods for data collection, image processing, 3D reconstruction and model building
- hands-on experience in preparing samples for cryo-EM analysis
- hands-on experience in operating the cryo-EM
- hands-on experience in using various computational methods to process data and generate atomic models

Innehåll

- Transmission electron microscope optics
- Fourier transforms and image formation in transmission electron microscopy
- Principles of biological sample preparation and optimization for cryo-TEM
- Electron microscopy operations and methods for data collection
- Principle methods of cryo-EM image processing, 3D reconstruction, map validation and model building

Undervisning

- Lectures
- Labs (wet labs and computer labs)
- Student presentations
- Student-led seminars

Examination

- Project presentation

Lärare

Daniel Larsson, Guillaume Gaullier

Kontakt

Daniel Larsson, daniel.larsson@icm.uu.se

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