Odling av celler från människa - metoder och tillämpningar


Kursnummer47
År2021
TypMethodcourse
SpårInflammation, Metabolism,
Max antal deltagare12
Sista ansökningsdag2021-05-01
SpråkEn
KursansvarigPatrik Lundquist
InstitutionInstitutionen för farmaci
BesöksadressBMC, Husargatan 3, byggnad B3
PostadressBox 580, 751 23 , Uppsala
Datum31 maj - 4 juni 2021
LokalMeddelas senare, på BMC/ zoom
Kurslängd1 vecka
Kursrapport 
Kursplan 

 

Beskrivning

A combined theoretical and practical course in cell culture methods focusing on culture of human primary cells and cell lines.

Inlärningsmål

Knowledge in the theory and practice of cell culture. Knowledge in basic and state-of-the-art methods applied to cell cultures. To have developed skills for bench work with cell cultures on an individual basis.

Innehåll

Cell culture reflecting stem, transit amplifying, differentiated and terminally differentiated tissue states; Monolayer and organotypic culture involving one or more cell types. Applicability of cell cultures as alternatives to laboratory animal experiments. Mechanisms regulating cell growth and viability, differentiation and apoptosis. Assessment of cell transformation to immortal and malignant phenotypes. Isolation of specific cells, e.g., epithelial cells, characterisation of cultured cells. Handling and sterile techniques, choice of materials and media for cell culture, e.g., serum-dependent vs. serum-free culture conditions. Cell cloning and gene transfer. Practical handling of cultures: thawing/freezing, passage, expansion and long-term storage. Handling of normal and tumor tissue for optimizing obtainment of cultures. Tissue engineering practices. Transcriptomics, proteomics and informatics methods for biomedical research with cell lines. Discussion of participants own culture experience and problems.

Undervisning

Lectures, laboratory work, IT-use and group discussions on pitfalls and possibilities with cell cultures

Examination

Presence at scheduled lectures and time allocated for oral examination of all participants is compulsory.
Literature studies and oral presentation of results for the course participants. Based on documented participation in the lectures, the oral examination can be arranged separately with the course organizer.

Litteratur

Handouts are provided for all elements of the course.

Lärare

Roland Grafström
Patrik Lundquist

Kontakt

Patrik Lundquist
Department of Pharmacy
Husargatan 3 (B3:3)
Box 580
751 23 Uppsala

patrik.lundquist@farmaci.uu.se

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