Theoretical introduction to laboratory methods in pre-clinical research (3 HP)

 
15
2019
Subject course
Spår
20
2019-10-15
English
Malin Grönberg, Azita Monazzam, Su-Chen Li, Elham Barazeghi, Maria João Pereira, Masoud Razmara
Medical sciences
Akademiska sjukhuset, ing 40, 5 tr
Akademiska sjukhuset, 751 85 Uppsala
Weeks 46-47
Rudbeck laboratory
2 weeks
 

Description
The course aims to introduce pre-clinical research methods in medical science and to give a broad knowledge about their practical use in planning, execution, analysis and problem-solving in research. After finishing the course, the student will have an overall understanding of common techniques in pre-clinical research, their applications, interpretation of results and will be able to choose which methods that are relevant to test a specific hypothesis.

Learning outcomes
After completing the course, the students are expected to be able to describe the pre-clinical techniques presented in the course, i.e. to be able to explain their theoretical background and possible applications.

Knowledge and understanding
After the completed course, the student is expected to
-Have a general understanding of the theoretical basics for modern laboratory methods in medical science and their possible applications.

Skill and ability
After the completed course, the student is expected to
-Show the ability to choose the right technique to answer a given research question.
-Describe the limitations of different laboratory methods.
-Show the ability to interpret the experimental results.

Contents
The course will give a theoretical overview of a few widely used laboratory techniques and includes:
-Introduction to research methodology
-Planning: before you start a research project
-Data management
-Cell- and tissue cultures from human and animal models.
-DNA and RNA isolation
-Gene editing with CRISPR/Cas9
-PCR, RT-PCR, real-time PCR
-Protein isolation, immunoprecipitation and Western blot
-Immunohistochemistry and electron microscopy
-Glucose uptake and lipolysis
-Pre-clinical Positron emissions tomography (PET) techniques

Instructions
The teaching will, to a large extent, be done by desk but will also include group/own projects that will be presented for the teachers and the other student. The course will consist of lectures, discussions and seminars.
The students are expected to attend all lectures and seminars. Attendance in the lectures and seminars are mandatory. In case of absence, the student needs to compensate by further work.

Examination
The examination of the course consists of fulfilling the compulsory session, this includes:
-Presence in all lectures and seminars.
-At the beginning of the course, the students will be divided into groups and provided a case. During the week, they will investigate how to solve each case with the current techniques. A short report will be submitted to the course leaders. The cases are then presented briefly during the last day and discussed between the groups with the course leaders as discussion leaders.
-All students have to actively participate in the seminar/discussion.

Literature
Reviews, articles and compendium.

Teachers
Course leaders.

Contacts
Malin Grönberg, PhD, research scientist at IMV; Endocrine oncology, malin.gronberg@medsci.uu.se

Azita Monazzam, PhD, research scientist at IMV; Endocrine tumor biology, azita.monazzam@medsci.uu.se

Su-Chen Li, PhD, post doctoral fellow at IMV; Endocrine tumor biology, Su-Chen.Li@medsci.uu.se

Elham Barazeghi, PhD, post doctoral fellow at Department of Surgical Sciences, Endocrine Surgery, elham.barazeghi@surgsci.uu.se

Maria João Pereira, PhD, post doctoral fellow at IMV; Clinical diabetology and metabolism, maria.pereira@medsci.uu.se

Masoud Razmara, PhD, biomedical analyst at IMV; Endocrine tumor biology, masoud.razmara@medsci.uu.se

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