Advanced Molecular Technology and Instrumentation for Proteome Analyses
| Course number | 13 |
| Year | 2019 |
| Type | Methodcourse |
| Track | - |
| Max participants | 25 |
| Application deadline | 2019-06-15 |
| Language | En |
| Course leader | Assoc. Prof.Masood Kamali-Moghaddam |
| Department | Department of Immunology, Genetics and Pathology |
| Visiting address | BMC, C10:2 |
| Post address | 751 85 Uppsala |
| Dates | Date: 2019-08-19 till 2019-08-30 |
| Location | BMC and Rudbeck Laboratory |
| Course length | 12 days |
| Course report | |
| Course plan |
Description
The course will provide insight into state-of-the-art technologies for proteome analyses in research and medicine via advanced molecular technologies, reagent resources and instrumentation. The course will be organized by the proteomics facilities at Science for Life Laboratory, Uppsala, and will convey, by lectures and hands-on training, information about established and emerging methods and technologies relevant in basic research and diagnostics. Affinity- and non-affinity-based methods for protein analyses will be covered.
Learning outcomes
The aim of the course is to introduce scientists at the Disciplinary domains of Medicine and Pharmacy and of Science and Technology and other interested scientists to novel and advanced technologies for proteome analysis offering powerful tools in basic research and diagnostics. The course is intended to facilitate networking between scientists and these proteomics facilities at the SciLifeLab-Uppsala. Both affinity- and non-affinity-based methods for protein detection will be covered. Mass spectrometry will be used for unbiased and high-throughput protein discovery and characterization; different variants of proximity ligation assays (PLAs) will be used for sensitive detection of proteins and their interactions and modifications in solution or in situ; and the participants will be introduced to histopathology methods including tissue microarray production, immunohistochemistry and slide scanning, using techniques, reagents and sample resources from the Human Proteome Atlas project.
Contents
The theoretical moments will include lectures, seminars and discussions, and they will be combined with practical moments including introduction to mass spectrometry, solid phase- and in situ PLA, and immunohistochemistry with tissue microarray production and image analysis.
Instructions
Examination
Full-time attendance of the course.
Literature
Reviews, original articles and laboratory manuals.
Teaching staff
Associate Prof. Masood Kamali-Moghaddam
Prof. Ulf Landegren
Dr. Radiosa Gallini
Dr. Alireza Azimi
Dr. Dr. Cecilia Lindskog Bergström
Dr. Per-Henrik Edqvist
Prof. Jonas Bergquist
Associate Prof. Sara Lind
Dr. Ganna Shevchenko
Alexander Falk
et al.
More information
Masood Kamali-Moghaddam
Dept. of Immunology, Genetics and Pathology
Rudbeck Laboratory
E-mail: masood.kamali@igp.uu.se
Tel: 018-471 4454 (office), 070-7454366 (mobile)