Advanced Molecular Technology and Instrumentation for Proteome Analyses

 
13
2019
Method course
25
2019-06-15
English
Assoc. Prof.Masood Kamali-Moghaddam
Department of Immunology, Genetics and Pathology
BMC, C10:2
751 85 Uppsala
Date: 2019-08-19 till 2019-08-30
BMC and Rudbeck Laboratory
12 days
 

The course will provide insight into state-of-the-art technologies for proteome analyses in research and medicine via advanced molecular technologies, reagent resources and instrumentation. The course will be organized by the proteomics facilities at Science for Life Laboratory, Uppsala, and will convey, by lectures and hands-on training, information about established and emerging methods and technologies relevant in basic research and diagnostics. Affinity- and non-affinity-based methods for protein analyses will be covered.

The aim of the course is to introduce scientists at the Disciplinary domains of Medicine and Pharmacy and of Science and Technology and other interested scientists to novel and advanced technologies for proteome analysis offering powerful tools in basic research and diagnostics. The course is intended to facilitate networking between scientists and these proteomics facilities at the SciLifeLab-Uppsala. Both affinity- and non-affinity-based methods for protein detection will be covered. Mass spectrometry will be used for unbiased and high-throughput protein discovery and characterization; different variants of proximity ligation assays (PLAs) will be used for sensitive detection of proteins and their interactions and modifications in solution or in situ; and the participants will be introduced to histopathology methods including tissue microarray production, immunohistochemistry and slide scanning, using techniques, reagents and sample resources from the Human Proteome Atlas project.

The theoretical moments will include lectures, seminars and discussions, and they will be combined with practical moments including introduction to mass spectrometry, solid phase- and in situ PLA, and immunohistochemistry with tissue microarray production and image analysis.

Full-time attendance of the course.

Reviews, original articles and laboratory manuals.

Associate Prof. Masood Kamali-Moghaddam
Prof. Ulf Landegren
Dr. Radiosa Gallini
Dr. Alireza Azimi
Dr. Dr. Cecilia Lindskog Bergström
Dr. Per-Henrik Edqvist
Prof. Jonas Bergquist
Associate Prof. Sara Lind
Dr. Ganna Shevchenko
Alexander Falk
et al.

Masood Kamali-Moghaddam
Dept. of Immunology, Genetics and Pathology
Rudbeck Laboratory

E-mail: masood.kamali@igp.uu.se
Tel: 018-471 4454 (office), 070-7454366 (mobile)

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