Chemical Proteomics 2017, 1 ECTS credit (location: Solna)


Kursnummer60,03
År2017
TypSubjectcourse
Spår-
Max antal deltagare40
Sista ansökningsdag2017-09-22
SpråkEn
KursansvarigMassimiliano Gaetani, Roman Zubarev
InstitutionSciLifeLab
BesöksadressTotmtebodavägen 23, Solna
PostadressBox 1031, 171 21 Solna, Sweden
Datum2017-11-07 to 2017-11-09
LokalTBA
Kurslängd3 days
Kursrapport 
Kursplan 

 

Beskrivning

National course open for PhD students (prioritized), postdocs, researchers and other employees in need of skills of proteome-wide proteomics with aims in drug discovery and development, from all Swedish universities.

Inlärningsmål

This course will cover most updated chemical proteomics strategies available for identifying proteome-wide compound-target interactions, which ChemProt provides at the state-of-the-art level.

Innehåll

Topics will include:

Introduction on mass spectrometry-based proteomics approaches used to identify drug/compound target molecules, by using protein extracts and cultured cells.
Thermal protein profiling (TPP): how to benefit from ligand inducted changes of protein thermal stability to probe potential interactions using entire cells and protein extracts.
Functional identification of target by expression proteomics (FITExP): how to benefit directly from quantitative, most relevant and specific responses of cell proteome to drug treatment for the identification of targets and potential keys of the drug´s efficacy.
Hydrogen/Deuterium (H/D) exchange mass spectrometry (HDX MS): how to elucidate the interaction interface and map the binding site of a drug on a particular protein by H/D exchange and mass spectrometry.

Undervisning

Applications are only accepted via the course webpage:

https://www.scilifelab.se/events/chemprot_ht17/

Course fee: 1300 SEK.

Entry requirements:
Knowledge of basic in vitro assays using cells for drug/compound testing
Basic knowledge in experimental design and statistical methods
Basic knowledge in proteomics analysis
Practical interest into unbiased identification and analysis of the interaction between a drug/compound of interest and its protein target(s), and/or into unbiased discovery of the key proteins and their related mechanism of cell responses upon drug/compound treatment.

Examination

Participation during all parts of the course.

Litteratur

Will be provided during the course.

Lärare

Massimiliano Gaetani, Roman Zubarev, Mikhail Savitski, Janne Lehtiö and Rozbeh Jafari

Kontakt

https://www.scilifelab.se/events/chemprot_ht17/

david.gotthold@scilifelab.se

Visa alla kurser 2017 »